New Publication – MIPs specific for glycated haemoglobin in competitive pseudo-ELISA assays
Scientists at MIP Diagnostics Ltd., along with researchers at University of Leicester and Wroclaw University of Science and Technology, have successfully developed nanostructured molecularly imprinted polymers (nanoMIPs) with high affinity and high selectivity for glycated haemoglobin. Their findings have recently been published in Sensors and Actuators B: Chemical (IF 6.4)
Glycated haemoglobin is a form of haemoglobin that is usually chemically linked to the sugar glucose. The formation of the sugar-haemoglobin linkage indicates the presence of excessive sugar in the bloodstream, often indicative of diabetes. MIPs specific for glycated haemoglobin would allow for the development of alternative quantitative sensors and diagnostic assays.
A solid-phase imprinting method proprietary to MIP Diagnostics Ltd. was used to prepare particles imprinted for glycated haemoglobin by using the whole protein as a template. Harvested nanoMIPs were then treated through a negative selection process to obtain high affinity MIPs able to recognize glycated haemoglobin but without any cross-reactivity towards non-glycated haemoglobin. Both bulk and selected particles were tested in competitive pseudo-ELISA assays with haemoglobin and glycated haemoglobin as free targets. In both cases, a linear response was observed from 10-3 nM to 1 nM. After negative selection, MIPs showed no response to non-glycated haemoglobin together with an improvement of the linear range of the assay. It also allowed quantification of glycated haemoglobin in human serum with recoveries above 95 %.
If you are interested in reading the article by Katarzyna Smolinska-Kempisty, Antonio Guerreiro, Joanna Czulak, and Sergey Piletsky it can be found at the link below:
If you are interested in creating MIPs or nanoMIPS for your own assays contact MIP Diagnostics Ltd. below or at firstname.lastname@example.org.